DIG-HIGH PRIME

DIG-High Prime-labeled DNA probes has been used in a variety of hybridization techniques :

• in Southern blots
• in northern blots
• in dot/slot blots
• for screening of gene libraries
• in In situ hybridizations

Due to highly specific and sensitive detection systems, DIG-labeled DNA probes can be used for single-copy gene detection in 1μg total human DNA. The use of the alkali-labile form of DIG-dUTP, in which the digoxigenin moiety is connected to the spacer arm via an alkali-labile ester bond, enables easier and more efficient stripping and reprobing of blots.