Methylation of lysine residues in core histones plays a critical role in regulating gene expression.1 Jumonji domain containing 2E (JMJD2E) catalyzes the demethylation of histone H3 at lysince residue 9.2 Like other JmjC protein hydroxylase family members, JMJD2E is an α-ketoglutarate-dependent Fe (II) oxygenase.3 Purification of Fe-dependent JmjC family members by IMAC can result in displacement of the catalytic iron and decreased activity, therefore this Strep-tagged protein is purified by affinity chromatography using Strep-Tactin coated resin.4 Because of their implication in cancer cell growth, jumonji C domain-containing histone demethylase inhibitors may have the capacity to be anticancer agents.1WARNING This product is not for human or veterinary use.
