Prostaglandin F2α (PGF2α) is one of the five primary prostaglandins derived enzymatically directly from the endoperoxide PGH2. PGF2α was initially discovered in seminal fluid, and to date the majority of the functional roles ascribed to it relate to fertility, pregnancy, and parturition. PGF2α is a potent luteolytic agent and is used to induce ovulation in domestic livestock. It is also a potent uterine stimulant, and is part of the cascade of myometrial stimulants which induce and sustain labor. PGF2α is rapidly metabolized to 13,14-dihydro-15-keto PGF2α in vivo, by the enzymes 15-hydroxy prostaglandin dehydrogenase and Δ13-reductucase.1,2,3,4 Measurement of 13,14-dihydro-15-keto PGF2α in plasma can be used as a marker of the in vivo production of PGF2α.5,6 The Cayman Chemical 13,14-dihydro-15-keto PGF2α Enzyme Immunoassay is a competitive assay that provides measurements of 13,14-dihydro-15-keto PGF2α within the range of 2.3-5,000 pg/ml, typically with a detection limit (80% B/B0) of approximately 15 pg/ml. Although the tracer and antiserum for this assay have been sold separately for many years, the reagents are now combined with an accurate standard and packaged together in a convenient assay kit. The assay has been validated for the measurement of 13,14-dihydro-15-keto PGF2α in plasma, a common matrix for measurement of this PGF2α metabolite.5Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.WARNING This product is not for human or veterinary use.
