The DYKDDDDK epitope is an expression tag commonly used in protein expression experiments. Compared to its corresponding native protein, a DYDDDDK-tagged protein will migrate at a larger size on SDS-PAGE, which is equivalent to the size of the epitope tag. The size of the DYDDDDK epitope may vary depending on the expression vector being utilized, but usually ranges from 3 to 5 kDa. Cayman Chemical’s DYDDDDK polyclonal antibody will recognize proteins expressed with both N-terminal and C-terminal DYDDDDK tags.
