Anti-CD137 antibody produced in goat is suitable for the following applications:
- Agonist Activity: Measured by its ability to costimulate IFNg secretion by human T cells in the presence of anti-CD3. The ED50 for this effect is typically 5-20μg/mL.
- ELISA capture: It can be used as a capturing reagent in a human 4-1BB sandwich immunoassay in combination with biotinylated human 4-1BB detection antibody and recombinant human 4-1BB as the standard. The suggested coating concentration range is 0.2-0.8μg/ml. The recommended concentration of the detection antibody is 0.1-0.4μg/mL.
- Flow cytometry: 2.5μg/106 cells using Human T cells treated with PHA (phytohemagglutinin).
- Immunoblotting: A working concentration of 0.1μg/ml was determined using recombinant human CD137.
- Immunocytochemistry: 5-15μg/mL using immersion fixed human peripheral blood mononuclear cells.
