(±)8(9)-EET-d11 is intended for use as an internal standard for the quantification of (±)8(9)-EET (Item No. 50351) by GC- or LC-MS. (±)8(9)-EET is a racemic mixture of the R/S enantiomeric forms biosynthesized from arachidonic acid (Item Nos. 90010 | 90010.1 | 10006607).1,2 (±)8(9)-EET is a major cytochrome P450 (CYP) metabolite in the renal cortex.3 It activates PPARα in HEK293 cells when used at a concentration of 1 µM but inhibits NF-κB activity induced by IL-1β in a PPARα-dependent and -independent fashion.4 It is also a substrate of COX-1 and COX-2, as measured by oxygen consumption and product formation assays when used at a concentration of 50 µM.5 (8S,9R)-EET (1 µg/kg), but not (8R,9S)-EET, reduces the glomerular filtration rate (GFR) through COX-dependent pre-glomerular vasoconstriction in rats.6WARNING This product is not for human or veterinary use.
