C16 Dihydroceramide is a dihydroceramide compound relevant to the study of ceramide synthase activity and sphingolipid generation. The dihydroceramides are precursors to ceramides in the biosynthesis of glycosphingolipids and sphingomyelin, and C16 Dihydroceramide presents one dihydroceramide which may be useful in studying and monitoring sphingolipid biosynthesis and the effects of perturbing this pathway upon other biological events.