(+)-D-
threo-PDMP is a ceramide analog and is one of the four possible stereoisomers of PDMP .
1 (+)-D-
threo-PDMP is an inhibitor of glucosylceramide synthase.
2,3 It inhibits glucosylceramide synthase by 50% when used at a concentration of 5 μM in an enzyme assay.
3 (+)-D-
threo-PDMP is the active component of racemic DL-
threo-PDMP .
In vitro, (+)-D-
threo-PDMP inhibits the synthesis of glucosylceramide synthase and lactosylceramide in B16 melanoma cells when used at a concentration of 25 μM and inhibits cell binding to laminin and collagen when used at concentrations of 10 and 25 μM.
4 It also inhibits β-1,4-galactosyltransferase 6 (B4GALT6) and prevents lactosylceramide synthesis, which is a promotor of neuroinflammation in mice during chronic experimental autoimmune encephalomyelitis (EAE), a model of multiple sclerosis.
5 (+)-D-
threo-PDMP inhibits ganglioside biosynthesis, reduces long-term potentiation (LTP) in mouse hippocampal CA1 neurons, and impairs learning in the four-pellet taking test in mice.
6
