H371：장기(또는, 영향을 받은 알려진 모든 장기를 명시)에 손상을 일으킬 수 있음(노출되어도 특정 표적장기 독성을 일으키지 않는다는 결정적인 노출경로가 있다면 노출경로를 기재)
예방조치문구
P260：분진·흄·가스·미스트·증기·...·스프레이를 흡입하지 마시오.
P264：취급 후에는 손을 철저히 씻으시오.
P264：취급 후에는 손을 철저히 씻으시오.
P270：이 제품을 사용할 때에는 먹거나, 마시거나 흡연하지 마시오.
P405：밀봉하여 저장하시오.
P501：...에 내용물 / 용기를 폐기 하시오.
KAPA2G Robust HotStart ReadyMix 화학적 특성, 용도, 생산
용도
KAPA2G Robust HotStart? ReadyMix? has been used in:
PCR
Real time RT-PCR
Nested Polymerase Chain Reaction (nPCR)
Amplification of GC- or AT-rich templates
Templates containing common PCR inhibitors e.g. salts, urea, SDS, or ethanol
Crude sample PCR e.g. buccal swabs, cultured mammalian, yeast, or bacterial cells
Colony PCR
The KAPA2G Robust HotStart ReadyMix may be combined with KAPA Express Extract for fast and efficient DNA extraction and PCR. For more information, refer to the application notes for:
Blood PCR
Mammalian DNA barcoding
Fish DNA barcoding
FFPE PCR
일반 설명
The second-generation KAPA2G Robust DNA Polymerase was evolved to solve inconsistent amplification across a broad range of amplicon types (GC- and AT-rich). KAPA2G Robust HotStart? ReadyMix? enables higher processivity and specific activity, which translates to robust PCR performance, high sensitivity, and improved tolerance to common inhibitors. The high performance of the KAPA2G Robust DNA Polymerase is ideally suited for challenging PCR applications and difficult samples, eliminating the need for optimization using multiple enzymes and protocols.
Biochem/physiol Actions
DNA fragments generated with KAPA2G Robust HotStart? DNA Polymerase have the same characteristics as DNA fragments generated with wild-type Taq DNA polymerase and may be used for routine downstream analyses or applications, including restriction enzyme digestion, cloning, and sequencing. Like wild-type Taq, KAPA2G Robust HotStart has 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′→5′ exonuclease (proofreading) activity. The fidelity of KAPA2G Robust HotStart is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated. PCR products generated by KAPA2G Robust HotStart are 3′-dA-tailed and may be cloned into TA cloning vectors.
