Dissolve in water to make a 0.1M solution. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.
Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature. From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C. From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.
If a different concentration is desired, note that there is a concentration effect on the pH of the solution.
