indigoidine

indigoidine 화학적 특성, 용도, 생산

• 개요

  Indigoidine is a blue-violet organic pigment related to the Azaquinones group and synthesized by bacterial strains. It is biosynthesized by bacterial species such as Streptomyces chromofuscus，E. coli，and Corynebacterium insidiosum (Gangulyet al. 2019). It is used as a food colorant in cereal, baking, and ice-cream industries.

• 용도

  Indigoidine natural blue dye is a promising alternative to the synthetic dyes used to colored jeans, leather, food, beverages, cosmetics and paper. The blue color of the indigoidine compound is bright and sustainable. As a natural dye, it has promising health benefits as an antioxidant and antimicrobial actor. Because of a new purification process patented with the compound, indigoidine natural blue dye is safe to use in food and drinks.

• 정의

  ChEBI: Indigoidine is a member of the class of pyridone that is a dimeric blue pigment biosynthesised from L-glutamine. It has a role as a bacterial metabolite and a biological pigment. It is a pyridone, a ring assembly, a dicarboximide and an enamine. It derives from a L-glutamine.

• Biosynthesis

  Indigoidine is a water-insoluble blue pigment that was first isolated from phytopathogenic Erwinia as a powerful radical scavenger that enables phytopathogens to tolerate oxidative stress, organic peroxides, and superoxides during the plant defense response due to its structure of carbon–carbon double bonds conjugated with a carbonyl group. This bacterial pigment shows a bright blue color similar to that of indigo. Several different strains are reported to produce it. Indigoidine is assembled from two units of L-glutamine by a nonribosomal peptide synthetase (e.g. IndC from Erwinia chrysanthemi and Streptomyces aureofaciens CCM 3239, BpsA from Streptomyces lavendulae and Sc-indC from Streptomyces chromofuscus ATCC 49982) (Figure 1.7b). Recently, an indigoidine biosynthetic gene cluster was located in the genome of S. chromofuscus ATCC 49982. The gene cluster is silent and consists of five open reading frames, called orf1, Sc-indC, Sc-indA, Sc-indB, and orf2. Sc-IndC was functionally characterized as an indigoidine synthase through heterologous expression of the enzyme in both Streptomyces coelicolor CH999 and E. coli BAP1. The titer of indigoidine in E. coli BAP1 was reported to be 2.78 g/L under optimized conditions. Its production was dramatically increased (by 41.4%/3.93 g/L) when Sc-IndB was co-expressed with it in E. coli BAP1 (Yu et al. 2013). In order to further improve production, a glutamine synthetase gene was amplified from E. coli and co-expressed with Sc-indC and Sc-indB in E. coli BAP1. At 2.5 mM (NH4)2HPO4, the titer can reach 7.08±0.11 g/L (Xu et al. 2015). This provides a green, efficient production process for this promising blue dye.

• Solubility in water

  Indigoidine is found in two redox states, an oxidized blue form which is insoluble in water and a reduced colorless form which is water soluble and historically referred to as leucoindigoidine[1].

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