Geneticin

한글명:Geneticin

카스 번호108321-42-2

상품명:Geneticin

CBNumberCB5852743

분자식C20H42N4O14S

포뮬러 무게594.63

MOL 파일108321-42-2.mol

화학적 성질

저장 조건	2-8°C
용해도	H₂O: 50 mg/mL
물리적 상태	백색분말
색상	흰색에서 황백색까지
optical activity	[α]/D (Specific Rotation: +104.4o (c=0.3% in H2O at 26oC))
수용성	녹는
안정성	흡습성
InChIKey	QNQZPJLBGRQFDD-ZLRXUJDMSA-N

위험 및 안전 성명

위험품 표기	Xn,Xi
위험 카페고리 넘버	20/21/22-36/37/38-42/43
안전지침서	22-24/25-36/37-23-36-26-45
WGK 독일	3
RTECS 번호	CB9378500
HS 번호	29419090

그림문자(GHS)

Geneticin 화학적 특성, 용도, 생산

개요
Geneticin (also known as G418, G418 sulfate) is an aminoglycoside antibiotic similar in structure to gentamicin B1, produced by Micromonospora rhodorangea. Geneticin blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells and is commonly used in laboratory research to select genetically engineered cells. Resistance to Geneticin is conferred by the neo gene from Tn5 encoding an aminoglycoside 3'-phosphotransferase, APH 3'II.
화학적 성질
white to off-white powder
용도
Geneticin is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL. It is also used to membrane-permeant allosteric effector of hemoglobin therapeutic in cardiovascular diseases and cancer.
주요 응용
Geneticin's most common application is in molecular biology as a selection agent. Geneticin(G418 sulfate) is toxic to bacteria, yeast, protozoa, helminths, and mammalian cells. Resistance is conferred by one of two dominant genes of bacterial origin which can be expressed in eukaryotic cells. G418 is water soluble and can be stored at room temperature for as long as 1 year. Aqueous solutions should be stored frozen. The amount of G418 required for selection will vary with each cell type and growth cycle. Although cells that are multiplying will be affected sooner than those that are not, cells that are in log phase will still require 3 to 7 days for selection. In general, the amount of G418 required for selection of mammalian cells is 400 mg/ml and 200 mg/ml for maintenance.
일반 설명
The optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 μg/ml Plant cells: 10-100 μg/ml Yeast cells: 0.5-1.0 μg/ml Mammalian cells: 0.1-2.0 μg/ml
Mode of action
Geneticin works by blocking polypeptide synthesis by preventing the elongation step within eukaryotic cells.
Resistance: Resistance to Geneticin is conferred through the NPTll gene in plant cells and the NEO gene in mammalian cells, which encodes aminoglycoside 3'-phosphotransferase.
Action: Geneticin functions by causing a chain of nonsense mutations during translation, ultimately resulting in inhibition of polypeptide synthesis and protein elongation.

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