HUMAN FIBRINOGEN

한글명:HUMAN FIBRINOGEN

카스 번호9001-32-5

상품명:HUMAN FIBRINOGEN

CBNumberCB4683502

분자식C6H14O6

포뮬러 무게182.17176

MOL 파일9001-32-5.mol

화학적 성질

저장 조건	-20°C
용해도	0.9% NaCl: 가용성
물리적 상태	가루
색상	하얀색
EPA	Fibrinogens (9001-32-5)

위험 및 안전 성명

위험품 표기	B
WGK 독일	3
F 고인화성물질	10-21
TSCA	Yes

그림문자(GHS)

HUMAN FIBRINOGEN 화학적 특성, 용도, 생산

화학적 성질
White or grayish amorphous substance.
용도
Diagnostic aid (vascular patency); radioactive agent.
정의
A sterile fraction of normal human blood plasma, dried from the frozen state. In solution, it has the property of being converted into insoluble fibrin when thrombin is added. It is an essential factor in the blood-clotting mechanism.
일반 설명
Fibrinogen, or Factor I, is a blood protein that is involved in clotting and is converted to fibrin by thrombin. Fibrinogen has an approximate molecular weight of 340 kDa
Purification Methods
A likely impurity is plasminogen. It is purified by glycine precipitation [Mosesson & Sherry Biochemistry 5 2829 1966] to obtain fractions 1-2, then further purified [Blomb.ck & Blomb.ck Arkiv Kemi 10 415 1956] and contaminating plasminogen is removed by passage through a lysine-Sepharose column. Such preparations are at least 95% clottable as determined by Mosesson and Sherry's method (above ref.) in which the OD280 is measured before and after clotting with 5 Units/mL of thrombin (> 3000U/mg). All fibrinogen preparations are treated with calf intestinal alkaline phosphatase to convert any fibrinogen peptide-AP to fibrinogen peptide-A by removing serine-bound phosphate. Solutions are then lyophilised and stored at -20o. [Higgins & Shafer J Biol Chem 256 12013 1981.] It is sparingly soluble in H2O. Aqueous solutions are viscous with isoelectric point at pH 5.5. It is readily denatured by heating above 56o or by chemical agents, e.g. salicylaldehyde, naphthoquinone sulfonates, ninhydrin or alloxan. [Edsall et al. J Am Chem Soc 69 2731 1947, Purification: Cama et al. Naturwissenschaften 48 574 1961, Lorand & Middlebrook Science 118 515 1953, cf. Fuller in Methods Enzymol 163 474 1988.] For plasminogen-deficient fibrinogen from blood plasma, the anticoagulated blood is centrifuged and the plasma is frozen and washed with saline solution. It is treated with charcoal, freeze-thawed and dialysed versus Tris/NaCl buffer. [Maxwell & Nikel Biochemical Preparations 12 16 1968.]

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