H371：장기(또는, 영향을 받은 알려진 모든 장기를 명시)에 손상을 일으킬 수 있음(노출되어도 특정 표적장기 독성을 일으키지 않는다는 결정적인 노출경로가 있다면 노출경로를 기재)
예방조치문구
P260：분진·흄·가스·미스트·증기·...·스프레이를 흡입하지 마시오.
P264：취급 후에는 손을 철저히 씻으시오.
P264：취급 후에는 손을 철저히 씻으시오.
P270：이 제품을 사용할 때에는 먹거나, 마시거나 흡연하지 마시오.
P501：...에 내용물 / 용기를 폐기 하시오.
KAPA Taq HotStart 화학적 특성, 용도, 생산
용도
KAPA Taq HotStart? may be used in:
High throughput PCR
Amplification of low copy DNA templates
Multiplex PCR
Specific amplification of complex templates
RT-PCR
Polymerase chain reaction (PCR)
일반 설명
KAPA Taq is supplied in a 2X ReadyMix? format containing all the components required for PCR except primers and template-simply use PCR-grade water to make up the required reaction volume. KAPA Taq ReadyMix is also available with loading dye reaction buffer, allowing, you to load your PCR product directly onto the agarose gel with no extra steps for adding loading/tracking dye. KAPA Taq DNA polymerase is the single-subunit Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus, purified from recombinant Escherichia coli.
Biochem/physiol Actions
KAPA Taq and KAPA Taq HotStart? DNA Polymerase have 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′ → 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity. KAPA Taq hotStart buffer is a uniquely formulated buffer to facilitate specific primer annealing. This translates to higher yields of a specific product when compared to traditional Taq buffers, and improved amplification of GC- and AT-rich templates. However, KAPA Taq hotStart DNA polymerase may be used in combination with any standard Taq buffer with a pH of 8.3 or higher.
