시트레이트구문

시트레이트구문 화학적 특성, 용도, 생산

• 용도

  Citrate Synthase from porcine heart has been used:
  
  • to inject newt egg for determining its importance in egg activation at fertilization
  • in the preparation of reaction mix to determine pyruvate carboxylase enzyme activity
  • to examine whether it can induce a [Ca²⁺]?increase and egg activation in unfertilized eggs
  
  

• 정의

  An enzyme that catalyzes the condensation of oxaloacetic acid and acetyl-CoA to yield citric acid and coenzyme A.

• Biochem/physiol Actions

  Citrate synthase catalyses the conversion of citrate to acetyl-CoA in the presence of coenzyme-A with the release of H2O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine.

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