α-Chymotrypsin

한글명:α-Chymotrypsin

카스 번호9004-07-3

상품명:α-Chymotrypsin

CBNumberCB4167554

분자식N/A

포뮬러 무게0

MOL 파일Mol file

화학적 성질

녹는점	127°C
밀도	1.37[at 20℃]
증기압	0Pa at 25℃
저장 조건	-20°C
용해도	1mM HCl로 재구성합니다. 1mM HCl에 10mg/ml로 용해됩니다. 2mM 염화칼슘이 안정제 역할을 합니다. 분취된 용액을 -20°C에서 최대 일주일 동안 보관하세요.
물리적 상태	무염, 동결건조 분말
색상	하얀색
수용성	125g/L at 25℃
Merck	13,2282
LogP	-1.3 at 20℃
EPA	Chymotrypsin (9004-07-3)

위험 및 안전 성명

위험품 표기	Xn,B
위험 카페고리 넘버	36/37/38-42/43-42
안전지침서	26-36-36/37-24-22
WGK 독일	3
RTECS 번호	GC3050000
F 고인화성물질	3-10
TSCA	Yes
HS 번호	35079090
유해 물질 데이터	9004-07-3(Hazardous Substances Data)

그림문자(GHS)

α-Chymotrypsin MSDS

α-Chymotrypsin 화학적 특성, 용도, 생산

화학적 성질
Lyophilized powder, dialyzed
용도
α-Chymotrypsin from bovine has been used in a study to inform proteasome inhibition in order to advance anticancer research. α-Chymotrypsin from bovine has also been used in a study that functionalized surface anchored poly(methylhydrosiloxane) thin films on oxidized silicon wafers.
일반 설명
Chymotrypsin (Chymar) is extractedfrom mammalian pancreas and is used in cataractsurgery. A dilute solution is used to irrigate the posteriorchamber of the eye to dissolve the fine filaments that holdthe lens.
Mechanism of action
The general mechanism for chymotrypsin is the classic serine protease mechanism. Hydrolytic proteolysis by α-chymotrypsin begins with an initial nucleophilic attack on the peptide bond by Ser 195, activated by deprotonation by His 57. This leads to forming a tetrahedral intermediate, stabilized by the amide groups of Ser 195 and Gly 193. The subsequent collapse of this intermediate, assisted by protonation of the leaving group by His 57 and Asp 102, leads to an acyl-enzyme intermediate. Activation of a water molecule by His 57 and Asp 102 facilitates hydrolysis of this intermediate, resulting in the reformation of the catalytically active serine residue and the release of the product facilitated by protonation with His 57.
Purification Methods
α-Chymotrypsin is crystallised twice from four-tenths saturated ammonium sulfate solution, then dissolved in 1mM HCl and dialysed against 1mM HCl at 2-4o. The solution is stored at 2o [Lang et al. J Am Chem Soc 80 4923 1958].
참고 문헌
[1] R J Schilling, A K Mitra. “Degradation of insulin by trypsin and alpha-chymotrypsin.” Pharmaceutical Research 8 6 (1991): 721–7.

α-Chymotrypsin 준비 용품 및 원자재

α-Chymotrypsin 공급 업체

α-Chymotrypsin 관련 검색

Send Inquriy