엔비오마이신

엔비오마이신 화학적 특성, 용도, 생산

• 개요

  Enviomycin was found in the culture broth of Streptomyces griseoverticillatus var. tuberacticus by Toyo Jozo Co. in 1966 as a mixture of tuberactinomycins N and O. It is a water-soluble, basic peptide closely related to viomycin and showing selective activity against Mycobacterium species about twice as strong as that of viomycin. Enviomycin, in combination with other antituberculotic drugs, is used to treat tuberculosis caused by streptomycinresistant Mycobacterium.

• Originator

  Tuberactin,Toyo Jozo,Japan,1975

• Manufacturing Process

  Two liters of an aqueous medium consisting of glucose 3%, starch 2%, soybean meal 3% and sodium chloride 1.5% were equally divided and introduced into twenty 500-ml Erlenmeyer flasks, adjusted to pH 6, sterilized at 120°C for 30 minutes, inoculated with Streptomyces griseoverticillatus var. tuberacticus N6-130 and then rotatively shake-cultured (radius 2.5 cm, 330 rpm) at 30°C for 7 days, obtaining 1.5 liter of cultured broth containing 2,360 mcg/ml of tuberactinomycin-N.
  Filtered broth was passed at 2.5 ml/min through a resin column (2.5 cm diameter, 28 cm length) packed with 150 ml of ion exchange resin Amberlite IRC-50 sodium type (Rohm and Haas Co., USA.). The column was washed with water, eluted with 0.5N HCl at a flow rate 1.3 ml/min. The eluates were fractionated each 10 ml and tuberactinomycin-N activity was found at fractions No. 45-63 observed by ultraviolet absorption method and bioassay.
  The thus yielded active fraction, about 200 ml, was neutralized with sodium hydroxide, concentrated to about 15 ml in vacuo, separating the precipitated inorganic salts therefrom. After decolorization with active carbon, 150 ml of methanol was added, the mixture was allowed to stand overnight at 5°C and the precipitate was collected by filtration. The precipitate was washed withmethanol and dried in vacuo to yield crude tuberactinomycin-N hydrochloride (yield, 3.07 g; purity, 7 1.5%; recovery, 62%).

• Therapeutic Function

  Antitubercular

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