Protamine sulfate has been used in the transduction of
lentiviruses into human embryonic 293T cells
shRNA into mesenchymal stem cells/marrow stromal cells (MSC)
lenti Sca1-OCT4 plasmid into human cord blood hematopoietic progenitor CD34+ cells
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Protamine sulfate has an anticoagulanteffect, but if used in the proper amount, it counteractsthe action of heparin and is used as an antidote for thelatter in cases of overdosage. It is administered intravenouslyin a dose that depends on the circumstances.
