Ulex europaeus agglutinin, UEA-I

Ulex europaeus agglutinin, UEA-I 화학적 특성, 용도, 생산

• 용도

  General Western Blot Protocol:
  
  • Glycoprotein sample size: 500ng
  • Lectin Concentration: 0.1ug/ml
  
  
  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS page gel
  3. Transfer gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect using chemiluminescent substrate (CPS1-120)

• Biochem/physiol Actions

  Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.

Ulex europaeus agglutinin, UEA-I 공급 업체

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