Phenosafranin

그림문자(GHS)

• 그림문자(GHS)

  

• 신호 어

  Warning

• 유해·위험 문구

  H315：피부에 자극을 일으킴

  H319：눈에 심한 자극을 일으킴

  H335：호흡 자극성을 일으킬 수 있음

• 예방조치문구

  P261：분진·흄·가스·미스트·증기·...·스프레이의 흡입을 피하시오.

  P264：취급 후에는 손을 철저히 씻으시오.

  P264：취급 후에는 손을 철저히 씻으시오.

  P271：옥외 또는 환기가 잘 되는 곳에서만 취급하시오.

  P280：보호장갑/보호의/보안경/안면보호구를 착용하시오.

  P302+P352：피부에 묻으면 다량의 물로 씻으시오.

  P305+P351+P338：눈에 묻으면 몇 분간 물로 조심해서 씻으시오. 가능하면 콘택트렌즈를 제거하시오. 계속 씻으시오.

Phenosafranin 화학적 특성, 용도, 생산

• 화학적 성질

  green crystalline powder

• 용도

  Phenosafranin is a bacterial stain used in microscopy, biological stain, and nuclear staining.

• 정의

  ChEBI: Phenosafranine is an organic chloride salt having 3,7-diamino-5-phenylphenazin-5-ium as the counterion. It is commonly used for staining Gram negative bacteria red in smears to contrast with the blue Gram positive organisms. It has a role as a fluorochrome, a histological dye and a photosensitizing agent. It contains a 3,7-diamino-5-phenylphenazin-5-ium.

• Staining Procedures

  Phenosafranin is used to distinguish living from dead cells. A 0.1 % aqueous solution, it is taken up by dead or abnormal cells immediately, while living cells remain unstained for at least 30 min.

• Purification Methods

  Crystallise the chloride from dilute HCl. It has UV with at 530nm in H2O. The picrate decomposes on heating and has a solubility of 0.0048% in H2O at 18o. [Beilstein 23 H 395, Beilstein 25 H 394, 25 I 654, 25 II 338, 25 III/IV 3050.]

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