To confirm that a chimaeric monoclonal antibody had episolon chain, an elisa was performed using monoclonal anti-human IgE antibody (clone GE1) at a 1:1000 dilution.
일반 설명
Monoclonal Anti-Human IgE (mouse IgG2b isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulin E?(IgE) antibodies are tetramers with two light chains (κ or λ) and two ε-heavy connected by numerous intrachain disulfide bonds. IgE exists in two isoforms, one is associated with the B cell?receptor and the other being secreted by plasma cell. It has a short life span in plasma when compared to receptor bounded IgE.
Biochem/physiol Actions
Immunoglobulin E?(IgE) is implicated in acute allergic reactions and chronic inflammatory allergic?diseases, such as allergic asthma and chronic urticaria (CU). Receptor bounded IgE aids?antigen uptake and modulates the differentiation and proliferation of?B?cells.?It confers immunity against helminthic parasites. IgE is also involved in facilitating?type I hypersensitivity reactions. It regulates mast cell homeostasis and also exhibits immunomodulatory effects.
