For the labelling of DNA with radioactive dCTP using random oligonucleotides as primers. High Prime-labelled probes are used in a variety of hybridization techniques: Southern blots
Northern blots
Dot/slot blots
Screening of gene libraries
In situ hybridizations
High Prime has been used to label the probes by random hexamer labelling and labelling of probes with 32P by random priming.
일반 설명
High Prime, a novel labeling mixture, contains optimal concentrations of nucleotides and primers in a highly efficient reaction mix with Klenow enzyme. This convenient "all-in-one" principle of High Prime reduces pipetting steps to a minimum, and increases accuracy and reproducibility of labeling reactions.
