알파-케토글루타레이트탈수소효소

그림문자(GHS)

알파-케토글루타레이트탈수소효소 화학적 특성, 용도, 생산

• 용도

  α-Ketoglutarate Dehydrogenase from the porcine heart has been used:
  
  • to study the reversal of nitration by glutathione (GSH) in peroxynitrite-treated cells
  • to measure its activity by Spectramax M5 microplate spectrofluorimeter using heart mitochondria
  • as a positive control to evaluate its activity in by Spectramax GEMINI EM fluorescence microplate reader using mice neurons
  
  

• 일반 설명

  α-Ketoglutarate dehydrogenase (α-KGDH) is a multienzyme complex localized to the mitochondria. This integrated enzyme is made up of many units of thiamine pyrophosphate-dependent dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3), and dihydrolipoamide succinyl transferase (E2).

• Biochem/physiol Actions

  α-Ketoglutarate dehydrogenase (α-KGDH) is a key enzyme of bioenergetic processes and a controlling unit of metabolic flux through the Krebs cycle or tricarboxylic acid (TCA) cycle. It catalyzes the oxidative decarboxylation of α-ketoglutarate (KG) to succinyl-CoA by releasing reduced nicotinamide adenine dinucleotide (NADH). It is the rate-limiting reaction of the TCA cycle. This reaction contributes to the electrons of the respiratory chain and requires thiamine pyrophosphate as a cofactor. The reduction of NAD (nicotinamide adenine dinucleotide) is observed to determine its reaction rate. α-KGDH from porcine has an optimum pH range of 6.6–7.4. This enzyme is inhibited by oxidative stress and results in a metabolic deficiency. However, α-KGDH is also known to produce reactive oxygen species (ROS) leading to oxidative stress. Defective or limited levels of α-KGDH cause several neurodegenerative diseases such as Alzheimer′s disease.

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