グルタチオンS-トランスフェラーゼ FROM EQUINE LIVER

グルタチオンS-トランスフェラーゼ FROM EQUINE LIVER 価格

グルタチオンS-トランスフェラーゼ FROM EQUINE LIVER 化学特性,用途語,生産方法

• 使用

  Glutathione S-Transferase from human placenta has been used in antigen microarray and informatics analysis.

• 一般的な説明

  Glutathione S-transferase (GST) is a major detoxification enzyme, and exists as multiple cytoplasmic and membrane-bound isozymes. These isozymes differ in their catalytic activity, as well as in their non-catalytic binding properties. Cytoplasmic isoforms of GST are encoded by five genes, namely α, θ, μ, σ and π. α, μ and π are the most abundant forms in mammals. Membrane bound GST forms are encoded by a single gene.

• 純化方法

  It is purified by affinity chromatography using a column prepared by coupling glutathione to epoxy-saturated Sepharose. After washing contaminating proteins, the pure transferase is eluted with buffer containing reduced glutathione. The solution is then concentrated by ultrafiltration, dialysed against phosphate buffer at pH ~7 and stored in the presence of dithiothreitol (2mM) in aliquots at <-20o. [Simons & Vander Jag Anal Biochem 52 334 1977.]

グルタチオンS-トランスフェラーゼ FROM EQUINE LIVER 生産企業

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