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Mycophenolate mofetil

CAS No.128794-94-5
Chemical Name:Mycophenolate mofetil
CBNumber:CB5663336
Molecular Formula:C23H31NO7
Formula Weight:433.49
MOL File:128794-94-5.mol

Mycophenolate mofetil Property

Melting point 95-96°C
Boiling point 637.6±55.0 °C(Predicted)
Density 1.222±0.06 g/cm3(Predicted)
storage temp. room temp
solubility DMSO: ≥15mg/mL
form powder
pka 5.6(at 25℃)
color white to beige
Merck 14,6327
Stability Stable for 2 years from date of purchase as supplied. Solutions in DMSO or ethanol may be stored at -20° for up to 1 month.
InChIKey RTGDFNSFWBGLEC-SYZQJQIISA-N
CAS DataBase Reference 128794-94-5(CAS DataBase Reference)
FDA UNII 9242ECW6R0
UNSPSC Code 41116107
NACRES NA.24

Safety

Hazard Codes :Xn
Risk Statements :22
Safety Statements :22-24/25
RIDADR :UN 3077 9 / PGIII
WGK Germany :3
RTECS :MP7746700
HS Code :29349990
Hazardous Substances Data :128794-94-5(Hazardous Substances Data)

NFPA 704:

	0
2		0

Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal wordDanger
Hazard statements H302-H360D-H372-H410
Precautionary statements P202-P260-P264-P273-P301+P312-P308+P313

Mycophenolate mofetil Price More Price(5)

Brand: Sigma-Aldrich(India)
Product number: SML0284
Product name : Mycophenolate mofetil
Purity: ≥98% (HPLC)
Packaging: 10MG
Price: ₹4687.23
Updated: 2022/06/14
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Brand: Sigma-Aldrich(India)
Product number: SML0284
Product name : Mycophenolate mofetil
Purity: ≥98% (HPLC)
Packaging: 50MG
Price: ₹15869.45
Updated: 2022/06/14
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Brand: Sigma-Aldrich(India)
Product number: PHR1451
Product name : Mycophenolate Mofetil
Purity: Pharmaceutical Secondary Standard; Certified Reference Material
Packaging: 1G
Price: ₹22516
Updated: 2022/06/14
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Brand: TCI Chemicals (India)
Product number: M2387
Product name : Mycophenolate Mofetil
Purity:
Packaging: 100MG
Price: ₹2000
Updated: 2022/05/26
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Brand: TCI Chemicals (India)
Product number: M2387
Product name : Mycophenolate Mofetil
Purity:
Packaging: 1G
Price: ₹7400
Updated: 2022/05/26
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Mycophenolate mofetil Chemical Properties,Usage,Production

Description Mycophenolate mofetil was launched in 1995 in the U.S.A., its first market worldwide, for the prevention of acute kidney transplant rejection in conjunction with other immunosuppressive therapy and to treat refractory acute kidney graft rejection. With improved oral absorption and bioavailability, mycophenolate mofetil is a prodrug of mycophenolic acid (MPA), a fermentation product of several Penicillium species. MPA is a selective, reversible, non-competitive inhibitor of inosinate dehydrogenase and guanylate synthetase. It inhibits the de now pathway of purine biosynthesis. MPA was found to have more potent antiproliferative effects on T and B lymphocytes than other cell types. Compared with other immunosuppressants, mycophenolate mofetil is reportedly superior due to its unique mechanism of action and excellent safety profile for long term use. Mycophenolate mofetil is being investigated clinically in the treatment of heart and liver transplantation rejection, asthma, in preventing coronary artery restenosis, and in treating rheumatoid arthritis.
Chemical Properties White Powder
Originator Roche (Switzerland)
Uses An immunosuppressant.
Uses Mycophenolate mofetil has been used to treat wild-type embryos for inhibiting nucleotide synthesis.
Uses For the prophylaxis of organ rejection in patients receiving allogeneic renal, cardiac or hepatic transplants. Mycophenolate mofetil should be used concomitantly with cyclosporine and corticosteroids.
Indications Mycophenolate mofetil (MMF, CellCept) is an ester prodrug of mycophenolic acid (MPA), a Penicillium-derived immunosuppressive agent that blocks de novo purine synthesis by noncompetitively inhibiting the enzyme inosine monophosphate dehydrogenase. MPA preferentially suppresses the proliferation of cells, such as T and B lymphocytes, that lack the purine salvage pathway and must synthesize de novo the guanosine nucleotides required for DNA and RNA synthesis.MPA has been used for decades as a systemic treatment for moderate to severe psoriasis. MMF was developed to increase the bioavailability of MPA.
Definition ChEBI: A carboxylic ester resulting from the formal condensation between the carboxylic acid group of mycophenolic acid and the hydroxy group of 2-(morpholin-4-yl)ethanol. In the liver, it is metabolised to mycophenolic acid, an immunosuppressant for which it is prodrug. It is widely used to prevent tissue rejection following organ transplants as well as for the treatment of certain autoimmune diseases.
Manufacturing Process The synthesis of Mycophenolic acid (Canonica L. Et al., Tetrahedron Letters, 1971, N 28, p.2691-2692)
By condensation of sodium diethylmalonate and 3-methylpent-3-en-2-on in ethanol was obtained 2,3-dimethyl-4,6-dioxocyclohexanecarboxilic acid ethyl ester, which was aromatised to 4,6-dihydroxy-2,3-dimethylbenzoic acid ethyl ester (melting point 115-116°C). By treatment with diazomethane or with CH3I and K2CO3 this compound was transformed into 2,4-dimethoxy-5,6- dimethylbenzoic acid ethyl ester (melting point 62-63°C). The hydrolysis of the ester group furnished the 2,4-dimethoxy-5,6-dimethylbenzoic acid (melting point 208-210°C), which was converted into the amide: carbamic acid 3-methoxy-4,5,6-trimethylphenyl ester (melting point 225-229°C). Treatment of the amide with t-butylhypochlorite in methylene dichloride yielded the corresponding N-chloroamide which was photolysed to the intermediate iminolactone and was immediately hydrolized to 5,7-dimethoxy- 4-methyl-3H-isobenzofuran-1-one.
This compound with hydriodic acid in acetic acid in the presence of red phosphorous at reflux yielded 5,7-dihydroxy-4-methyl-3H-isobenzofuran-1- one. Condensation of 6-bromo-4-methylhex-4-enoic acid methyl ester and 5,7-dihydroxy-4-methyl-3H-isobenzofuran-1-one with silver oxide in dioxane at room temperature yielded 6-(4,6-dihydroxy-7-methyl-3-oxo-1,3-dihydroisobenzofuran-5-yl)-4-methylhex-4-enoic acid methyl ester (36% yield). At last, monomethylation with diazomethane yield 6-(4-hydroxy-6-methoxy-7- methyl-3-oxo-1,3-dihydro-isobenzofuran-5-yl)-4-methylhex-4-enoic acid methyl ester, which was hydrolysed with aqueous sodium hydroxide to 6-(4- hydroxy-6-methoxy-7-methyl-3-oxo-1,3-dihydro-isobenzofuran-5-yl)-4- methylhex-4-enoic acid (Mycophenolic acid).
Mycophenolic acid may be obtained by the fermentation broth of Pennicillium brevicompactum. The synthesis of Mycophenolate mofetil (Patent U.S. 4,753,935). The mixture of Mycophenolic acid (32.0 g), thionyl chloride (25.0 ml) and DMF (0.3 ml) in dichloromethane (250 ml) was stirred at room temperature for 3 hours, after which the volatile components were removed under vacuum to afford mycophenolic acid chloride as an oil. The mycophenolic acid chloride oil was dissolved in dichloromethane (50.0 ml) and added to the chilled solution of morpholinoethanol (30.5 ml) in dichloromethane (250 ml). After stirring for 90 min at 4°C, the reaction mixture was washed with water and then with aqueous sodium bicarbonate. The organic solution was dried with sodium sulfate and evaporated to yield Mycophenolate mofetil: morpholinoethyl E-6-(1,3-dihydro-4-hydroxy-6- methoxy-7-methyl-3-oxo-5-isobenzofuranyl)-4-methyl-4-hexenoate (melting point 93-94°C).
The product (38.0 g) was dissolved in isopropanol (200 ml) and the solution was added to a solution of hydrogen chloride (10.0 g) in isopropanol (150 ml). The hydrochloride of Mycophenolate mofetil was collected by filtration and dried under vacuum (melting point 154-155°C).
brand name CellCept
Therapeutic Function Antiarthritic, Immunosuppressive
Biochem/physiol Actions Mycophenolate mofetil is a prodrug of mycophenolic acid (Cat. # M5255) that is cleaved by nonspecific esterases in vivo to produce the parent compound. Mycophenolic acid blocks inosine monophosphate dehydrogenase and is a potent immunosuppresive agent.
Mechanism of action the guanosine nucleotides required for DNA and RNA synthesis.MPA has been used for decades as a systemic treatment for moderate to severe psoriasis. MMF was developed to increase the bioavailability of MPA.
Clinical Use MMF is indicated for the prophylaxis of organ rejection in patients receiving renal, hepatic, and cardiac transplants; it is often used in combination with other immunosuppressive agents for this indication. In dermatology, MMF is particularly useful as monotherapy, or as a steroid-sparing agent, for treatment of autoimmune blistering diseases (bullous pemphigoid and pemphigus). It may also be useful for the treatment of inflammatory skin diseases mediated by neutrophilic infiltration, such as pyoderma gangrenosum, and psoriasis.
Side effects Adverse effects produced by MMF most commonly include nausea, abdominal cramps, diarrhea, and possibly an increased incidence of viral and bacterial infections. Whether MMF may be associated with an increased long-term risk of lymphoma or other malignancies is controversial; however, any such risk is likely to be lower in patients treated for skin disease with MMF monotherapy than in transplant patients treated with combination immunosuppressive therapy.
Drug interactions Potentially hazardous interactions with other drugs
Antipsychotics: avoid with clozapine (increased risk of agranulocytosis).
Antivirals: higher concentrations of both mycophenolate and aciclovir or ganciclovir when the two are prescribed concomitantly
. Antacids: absorption of mycophenolate decreased in presence of magnesium and aluminium salts. Antibacterials: bioavailability of mycophenolate possibly reduced by metronidazole and norfloxacin; concentration of active metabolite reduced by rifampicin.
Colestyramine: 40% reduction in oral bioavailability of mycophenolate.
Ciclosporin: some studies show that ciclosporin decreases plasma MPA AUC levels; other studies show increases - no dose change required.
Iron preparations: may significantly reduce absorption of mycophenolate.
Sevelamer: reduced levels of mycophenolate.
Tacrolimus: increases MPA concentrations- no dose change required but monitor closely.
See 'Other information'
Metabolism Mycophenolate undergoes presystemic metabolism in the liver to active mycophenolic acid (MPA). MPA undergoes enterohepatic recirculation and secondary increases in plasma MPA concentrations are seen; these have been reported at between 6-12 hours after a dose of mycophenolate mofetil, and at between 6-8 hours after a dose of mycophenolate sodium. MPA is metabolised by glucuronidation to the inactive mycophenolic acid glucuronide. The majority of a dose of mycophenolate is excreted in the urine as this glucuronide, with negligible amounts of MPA; about 6% of a dose is recovered in faeces.
storage Store at RT
References 1) Allison and Eugui (1996), Purine metabolism and immunosuppressive effects of mycophenolate mofetil (MMF); Clin. Transplant., 10 77 2) Jonsson et al. (2002), Mycophenolic acid inhibits inosine 5′-monophosphate dehydrogenase and suppresses production of pro-inflammatory cytokines, nitric oxide, and LDH in macrophages; Cell. Immunol., 216 93 3) Allison et al. (1993), Mechanisms of action of mycophenolic acid; Ann. NY Acad. Sci., 696 63 4) Quemeneur et al. (2002), Mycophenolic acid inhibits IL-2-dependent T cell proliferation, but not IL-2-dependent survival and sensitization to apoptosis; J. Immunol., 169 2747 5) Ebrahimi et al. (2012) Time dependent neuroprotection of mycophenolate mofetil; effects on temporal dynamics in glial proliferation, apoptosis, and scar formation; J. Neuroinflammation, 9 89