Acid chrome blue K

Uses

Acid chrome blue K is a metal indicator. The solution color of the LAMP (Loop-mediated isothermal amplification) reaction with ACBK (Acid chrome blue K) changes from red to blue based on a decrease in the Mg2+ concentration in the reaction solution[1].

Preparation

3-Amino-4-hydroxybenzenesulfonic acid diazotization, and 4,5-Dihydroxynaphthalene-2,7-disulfonic acid coupling.

Properties and Applications

red light navy blue. Soluble in water and ethanol. In concentrated sulfuric acid to purple, dilution after blue light pink; In concentrated nitric acid for dark orange to yellow brown. Dye aqueous solution and sodium hydroxide as yellow solution.

Standard	Ironing Fastness	Light Fastness	Fulling		Persperation Fastness	Soaping	Water
			Alkali	Acid
ISO	4-5	5	5	2-3	4-5	5	5

References

[1] Wang X, et al. Use of a novel metal indicator to judge loop-mediated isothermal amplification for detecting the 35S promoter. Anal Bioanal Chem. 2017 Feb;409(4):881-889. DOI:10.1007/s00216-016-0084-x