Description
C6 NBD ceramide is a biologically active fluorescent analog of short chain, membrane-
permeable ceramides. It is as effective as C6 ceramide in the inhibition of viral glycoprotein transport through the Golgi. C6 NBD ceramide has been used as a fluorescent substrate for the activity of UDP-
glucose:ceramide glucosyltransferase and to demonstrate the translocation of glucocerebroside and sphingomyelin from the Golgi to the plasma membrane.
Chemical Properties
Rust or Dark Yellow Solid
Uses
A vital fluoresent stain for the Golgi apparatus.
Definition
ChEBI: N-{6-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hexanoyl}sphingosine is an N-acylsphingosine that is sphingosine with the amino nitrogen converted into a 6-{[N-(7-nitrobenzo-2,1,3-oxadiazol-4-yl)amino]}hexananamido group. It has a role as a fluorescent probe.
References
[1] CHAZOTTE B. Labeling Golgi with fluorescent ceramides.[J]. Cold Spring Harbor protocols, 2012, 2012 8. DOI:
10.1101/pdb.prot070599[2] G VAN MEER. Sorting of sphingolipids in epithelial (Madin-Darby canine kidney) cells.[J]. Journal of Cell Biology, 1987, 105 4: 1623-1635. DOI:
10.1083/jcb.105.4.1623[3] ANTHONY S. DON Hugh R. A fluorescent plate reader assay for ceramide kinase[J]. Analytical biochemistry, 2008, 375 2: Pages 265-271. DOI:
10.1016/j.ab.2007.12.024[4] NIKOLOVA-KARAKASHIAN M. Methods to Characterize Synthesis and Degradation of Sphingomyelin at the Plasma Membrane and Its Impact on Lipid Raft Dynamics.[J]. Methods in molecular biology, 2021, 2187: 113-129. DOI:
10.1007/978-1-0716-0814-2\_7
