Uses
CY5-amino groups can be used as polar tracers, reactive dyes for labeling proteins via carboxylic acid moieties, and amine donors for enzymatic transamination labeling.
Description
Cyanine5 amine is a reactive dye which contains amino group, an analog of Cy5® amine. This reagent can be coupled with a variety of activated esters and other electrophilic reagents. For example, this amine can be coupled with EDC-activated carboxylic groups.
This bright and photostable dye is suitable for many different methods of fluorescence detection. Colorful fluorophore can also be easily detected in small quantities (nanomols) by naked human eye.
Chemical Properties
Appearance: Dark blue solid
ex/em: 640/664 nm (PBS buffer)
Extinction coefficient (ε): 250000 Lmol1cm1
Quantum yield (Φ): 0.2
Fluoresene quantum yield
0.2
Extinction Coefficient
250000
