Synthesis
The biosynthesis of Mogroside V involves five enzyme families: squalene epoxidases, triterpenoid synthases, epoxide hydrolases, cytochrome P450s, and UDP-glucosyltransferases. Specifically, the uridine diphosphate-dependent glycosyltransferases UGT720-269-1 and UGT94-289-3 from S. grosvenorii catalyze the glycosylation of mogrol in vitro to produce Mogroside V[1][2].
References
[1]Itkin, M., Davidovich-Rikanati, R., Cohen, S., Portnoy, V., Doron-Faigenboim, A., Oren, E., Freilich, S., Tzuri, G., Baranes, N., Shen, S., Petreikov, M., Sertchook, R., Ben-Dor, S., Gottlieb, H., Hernandez, A., Nelson, D. R., Paris, H. S., Tadmor, Y., Burger, Y., … Schaffer, A. (2016). The biosynthetic pathway of the nonsugar, high-intensity sweetener mogroside V from <i>Siraitia grosvenorii</i>. Proceedings of the National Academy of Sciences, 113(47).
https://doi.org/10.1073/pnas.1604828113 [2]Qu, G., Song, Y., Xu, X., Liu, Y., Li, J., Du, G., Liu, L., Li, Y., & Lv, X. (2025). De novo biosynthesis of mogroside V by multiplexed engineered yeasts. Metabolic Engineering, 88, 160–171.
https://doi.org/10.1016/j.ymben.2025.01.002
