Description
Cytidine 5'-monophosphate, also known as 5'-CMP, is a nucleotide that is used as a monomer in RNA. 5'-CMP is a key intermediate in the preparation of several nucleotide derivatives and is widely used in food and pharmaceutical industries.
Chemical Properties
white crystalline powder
Uses
Cytidine 5′-monophosphate has been used in Raman spectroscopy studies.
Uses
Cytidine 5'-monophosphate (5'-CMP) is a key intermediate in the production of nucleotide derivatives and has been widely used in the food, agriculture and pharmaceutical industries.
Definition
ChEBI: Cytidine 5'-monophosphate is a pyrimidine ribonucleoside 5'-monophosphate having cytosine as the nucleobase. It has a role as a human metabolite, an Escherichia coli metabolite and a mouse metabolite. It is a pyrimidine ribonucleoside 5'-monophosphate and a cytidine 5'-phosphate. It is a conjugate acid of a cytidine 5'-monophosphate(2-).
Production Methods
As a key intermediate in preparing several nucleotide derivatives, cytidine 5′-monophosphate (5′-CMP) is widely applied in food and pharmaceutical industries. Enzymatic synthesis of 5′-CMP is catalyzed by cytidine kinase (CK, EC 2.7.1.213), a type of nucleoside kinase that belongs to the PfkB family. Li et al. constructed a highly efficient biosynthesis system for manufacturing 5′-CMP in vitro. CK was used for the biotransformation of cytidine to 5′-CMP, while polyphosphate kinase (PPK) was coupled for adenosine triphosphate regeneration. Both CK and PPK were selected from extremophiles, possessing great potential for biocatalytic synthesis. After optimization, 96 mM 5′-CMP was produced within 6 h, and the yield reached nearly 100%[1].
benefits
Cytidine 5’-monophosphate (CMP) is a nucleotide supplement with potential benefits such as improved muscle function, immune support, and enhanced neuronal growth. CMP is a component of RNA and phospholipids and sometimes pairs with uridine and other nutrients to produce synergistic effects, particularly in brain health. Studies have shown that adding CMP as a supplement to fish feed can contribute to their growth, health performance, and immune response.
Biochem/physiol Actions
Cytidine 5′-monophosphate (CMP) is used as a substrate of uridine monophosphate (UMP)/cytidine monophosphate (CMP) kinase (EC 2.7.4.4) to form CDP which upon phosphorylation to CTP supports DNA and RNA biosynthesis.
Side effects
Cytidine 5’-monophosphate (CMP) is a naturally occurring substance and is considered relatively safe, with no adverse health effects or respiratory irritation. No serious adverse reactions have been reported when CMP is used as a supplement; the most common side effects are gastrointestinal reactions, including nausea, vomiting, diarrhea, abdominal pain, and decreased appetite. Headaches may occur with long-term use.
References
[1] Zonglin Li. “Efficient One-Pot Synthesis of Cytidine 5′-Monophosphate Using an Extremophilic Enzyme Cascade System.” Journal of Agricultural and Food Chemistry 68 34 (2020): 9188–9194.
