ChemicalBook > CAS DataBase List > 5,5′-Dithiobis(2-nitrobenzoic acid)

5,5′-Dithiobis(2-nitrobenzoic acid)

CAS No.69-78-3
Chemical Name:5,5′-Dithiobis(2-nitrobenzoic acid)
CBNumber:CB2215444
Molecular Formula:C14H8N2O8S2
Formula Weight:396.35
MOL File:69-78-3.mol

5,5′-Dithiobis(2-nitrobenzoic acid) Property

Melting point 240-245 °C (dec.)(lit.)
Boiling point 671.9±55.0 °C(Predicted)
bulk density 290kg/m3
Density 1.67
refractive index 1.6900 (estimate)
storage temp. Keep in dark place,Sealed in dry,Room Temperature
solubility ethanol: 8mg/mL
pka 1.57±0.25(Predicted)
form Yellow solid
color Pale Yellow to Light Yellow
Water Solubility Soluble in water and ethanol.
Merck 14,3551
BRN 1896821
Stability Stable. Incompatible with strong bases, strong oxidizing agents.
InChIKey KIUMMUBSPKGMOY-UHFFFAOYSA-N
CAS DataBase Reference 69-78-3(CAS DataBase Reference)
FDA UNII 9BZQ3U62JX
EPA Substance Registry System Benzoic acid, 3,3'-dithiobis[6-nitro- (69-78-3)
UNSPSC Code 12352200
NACRES NA.26

Safety

Hazard Codes :Xi
Risk Statements :36/37/38
Safety Statements :26-36
WGK Germany :3
RTECS :DG9650000
F :10
TSCA :Yes
HS Code :29309070

NFPA 704:

	1
2		0

Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal wordWarning
Hazard statements H315-H319-H335
Precautionary statements P264-P280-P302+P352+P332+P313+P362+P364-P305+P351+P338+P337+P313-P261-P280a-P304+P340-P305+P351+P338-P405-P501a

5,5′-Dithiobis(2-nitrobenzoic acid) Price More Price(24)

Brand: Sigma-Aldrich(India)
Product number: D8130
Product name : 5,5′-Dithiobis(2-nitrobenzoic acid)
Purity: ≥98%, BioReagent, suitable for determination of sulfhydryl groups
Packaging: 500MG
Price: ₹2200
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: D8130
Product name : 5,5′-Dithiobis(2-nitrobenzoic acid)
Purity: ≥98%, BioReagent, suitable for determination of sulfhydryl groups
Packaging: 1G
Price: ₹3970
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: D8130
Product name : 5,5′-Dithiobis(2-nitrobenzoic acid)
Purity: ≥98%, BioReagent, suitable for determination of sulfhydryl groups
Packaging: 5G
Price: ₹9660
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: D8130
Product name : 5,5′-Dithiobis(2-nitrobenzoic acid)
Purity: ≥98%, BioReagent, suitable for determination of sulfhydryl groups
Packaging: 10G
Price: ₹17820
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: D8130
Product name : 5,5′-Dithiobis(2-nitrobenzoic acid)
Purity: ≥98%, BioReagent, suitable for determination of sulfhydryl groups
Packaging: 25G
Price: ₹24258.83
Updated: 2022/06/14
Buy: Buy

5,5′-Dithiobis(2-nitrobenzoic acid) Chemical Properties,Usage,Production

Chemical Properties Crystalline
Uses Reagent for determination of sufhydryl groups.5,5'-dithiobis-(2-nitrobenzoic acid) is widely utilized as a non-fluorescent probe to quantify the number or the concentration of thiol groups in a sample. It acts as a cleavable cross-linking agent. It finds an important application as a sensitive reagent for determining free sulfhydryl content in proteins, peptides, and tissues. It is also employed in high-performance liquid chromatography (HPLC) to determine the alkylthiol content.
Uses 5,5′-Dithiobis(2-nitrobenzoic acid) is a water soluble reagent mainly used for the derivatization of sulfhydryl groups.
Uses 5,5′-Dithiobis(2-nitrobenzoic acid) is an membrane-impermeant thiol/disulfide exchange blocker. It is also used to quantify the number or concentration of thiol groups in a sample.
Definition ChEBI: An organic disulfide that results from the formal oxidative dimerisation of 2-nitro-5-thiobenzoic acid. An indicator used to quantify the number or concentration of thiol groups.
General Description 5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent. It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH. Thiolate anion (RS^-) reacts with Ellman′s reagent (DTNB^2-), to produce 2-nitro-5-thiobenzoate anion (TNB^2-) and one mixed disulfide (R-S-TNB^-). TNB^2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3.
Biological Activity Cell permeable: no', 'Primary Target
Useful in photometric determination of thiols', 'Product does not compete with ATP.', 'Reversible: no