Taurolithocholic Acid-d5

Description

Taurolithocholic acid-d4 (TLCA-d₄) is intended for use as an internal standard for the quantification of TLCA by GC- or LC-MS. TLCA is a taurine-conjugated form of the secondary bile acid lithocholic acid .¹ TLCA (75 μM) increases caspase-3 and -7 activity in Hep3B cells transfected with sodium taurocholate cotransporting peptide (NTCP), but not nontransfected Hep3B cells.² It has been used to induce cholestasis in ex vivo and in vivo animal models of hepatocellular cholestasis.^3,4 Serum levels of TLCA increase approximately 5-fold within two hours during an oral lipid tolerance test in humans.¹

Uses

Taurolithocholic acid-d4 (sodium) is the deuterium labeled Taurolithocholic acid (sodium salt). Taurolithocholic acid sodium salt, a potent cholestatic agent, is a potent Ca2+ agonist[1].

References

1. Schmid, A., Neumann, H., Karrasch, T., et al. Bile acid metabolome after an oral lipid tolerance test by liquid chromatography-tandem mass spectrometry (LC-MS/MS) PLoS One 11(2),e0148869(2016).
2. Rust, C., Wild, N., Bernt, C., et al. Bile acid-induced apoptosis in hepatocytes is caspase-6-dependent J. Biol. Chem. 284(5),2908-2916(2009).
3. Denk, G.U., Maitz, S., Wimmer, R., et al. Conjugation is essential for the anticholestatic effect of NorUrsodeoxycholic acid in taurolithocholic acid-induced cholestasis in rat liver Hepatology 52(5),1758-1768(2010).
4. Javitt, N.B. Cholestasis in rats induced by taurolithocholate Nature 210(5042),1262-1263(1966).