4-Nitro-3-(octanoyloxy)benzoic acid

Description

NOBA (55894-52-5) is a chromogenic substrate for phospholipases such as PLA2. Read absorbance at 425 nm.

Uses

4-Nitro-3-[(1-oxooctyl)oxy]benzoic acid is used to acrylate ε-amino groups of Lysine (L468895) in order to immobilize phospholipase A2 in snake venom.

References

[1] W CHO  F J K. Chromogenic substrates and assay of phospholipases A2.[J]. Methods in enzymology, 1991, 197: 75-79. DOI:10.1016/0076-6879(91)97134-k
[2] A simple assay for a human serum phospholipase A2 that is associated with high density lipoproteins
[3] V.L. BONFIM . Structural and functional characterization of myotoxin, Cr-IV 1, a phospholipase A2 D49 from the venom of the snake Calloselasma rhodostoma[J]. Biologicals, 2008, 36 3: Pages 168-176. DOI:10.1016/j.biologicals.2007.10.004