ZZW-115 (0.1-33 μM; 72 hours) is efficient in killing cancer cells, with an IC 50 in the range of 0.84 μM (ANOR) to 4.93 μM (HN14 ).
ZZW-115 (0-100 μM; 24-72 hours) is efficient to kill these tumor cells with an IC 50 in the range of 0.42 μM (Hep2G cells) to 7.75 μM (SaOS-2 cells).
ZZW-115 induces pancreatic cell death by necrosis and apoptosis. ZZW-115 treatment induces a decrease in ATP production and induces a ROS overproduction.
LDH release is significantly higher in ZZW-115-treated cells (MiaPaCa-2, 02-063, LIPC, Foie8b, and HN14 cells) than in control cells in a concentration-dependent manner. Similarly, caspase 3/7 activity is also greater in ZZW-115-treated cells. These experiments demonstrated that ZZW-115 exerted both pronecrotic and proapoptotic effects.
Cell Viability Assay
| Cell Line: | ANOR cells, MiaPaCa-2, 02-063, 01008, LIPC, 02136, HN01,01046, AOIPC, Foie8b, HN14 cells | < /tr>
| Concentration: | 0.1- 33 μM |
| Incubation Time: | 72 hours |
| Result: | Was efficient in killing cancer cells, with an IC 50 in the range of 0.84 μM (ANOR) to 4.93 μM (HN14). |
Cell Proliferation Assay
| Cell Line: | U87, A375, U2OS, SaOS-2, HT29, SK-CO-1, LS174T, H1299 and H358, HepG2, PC3, THP-1, Daudi, Jurkat and MDA-MB-231 cells |
| Concentration: | 0-100 μM |
| Incubation Time: | 24 or 72 hours |
| Result: | Was efficient to kill these tumor cells with an IC 50 in the range of 0.42 μM (Hep2G cells) to 7.75 μM ( SaOS-2 cells). |
![1-Piperazineethanamine, N,N-dimethyl-4-[3-[2-(trifluoromethyl)-10H-phenothiazin-10-yl]propyl]- Structure](https://www.chemicalbook.com/CAS/20200611/GIF/801991-87-7.gif)
