Chemical Properties
white to slightly beige fine crystalline powder
Uses
4-Hydroxybenzhydrazide is a reagent for the rapid automated determination of serum glucose.It can be used as an indicator for the determination of carbohydrates. The acid-hydrolyzable side chains of tissue (liver) bound 4-Hydroxybenzhydrazide can be used as markers to monitor its residues in chickens.
Uses
4-Hydroxybenzhydrazide has been used:
for determining the concentration of maltose from starch digestion
for analysis of sugars from fructan containing food hydrolysate
in colorimetric assay for reducing sugar estimation from processed feedstuff
Biochem/physiol Actions
4-Hydroxybenzhydrazide (PAHBAH), is used for assaying α-amylase and glucoamylase activities. The assay based on PAHBAH measures non-specifically the free reducing sugar groups present in samples at 415nm colorimetrically.
