Description
BDP 558/568 NHS ester is a BDP dye linker with similar emission to other dyes such as Cyanine3. The introduction of the NHS ester makes the compound more reactive with primary amines.
Chemical Properties
Appearance: Dark brown flaky solid
ex/em: 560/574 nm
Extinction coefficient (ε): 84400 Lmol1cm1
Quantum yield (Φ): 0.68
Uses
BDP 558/568 NHS ester is a borondipyrromethene fluorophore with emission in the yellow part of the spectrum. BDP 558/568 NHS ester is an amine reactive NHS ester, and the absorption and emission spectra of BDP 558/568 NHS ester are similar with TAMRA, BDP TMR, Cyanine3, and sulfo-Cyanine3[1][2].
References
[1] Vasselon T, et al. Internalization of monomeric lipopolysaccharide occurs after transfer out of cell surface CD14. J Exp Med. 1999 Aug 16;190(4):509-21. DOI:
10.1084/jem.190.4.509[2] Yu B, Wright SD. Catalytic properties of lipopolysaccharide (LPS) binding protein. Transfer of LPS to soluble CD14. J Biol Chem. 1996 Feb 23;271(8):4100-5. DOI:
10.1074/jbc.271.8.4100
Fluoresene quantum yield
0.68
Extinction Coefficient
84400
