Description
(Z-
Ala-
Ala-
Ala-
Ala)2Rh110 is a sensitive fluorogenic elastase substrate. The colorless and nonfluorescent (Z-
Ala-
Ala-
Ala-
Ala)2Rh110 is selectively cleaved by elastase to yield the highly fluorescent compound rhodamine 110, which can be analyzed with an excitation wavelength of 485 nm and emission wavelength of 525 nm.
in vitro
(z-ala-ala-ala-ala)2rh110 has been identified as a very sensitive fluorogenic elastase substrate. the colorless and nonfluorescent z-ala-ala-ala-ala)2rh110 was found to be selectively cleaved by elastase to yield the highly fluorescent compound rhodamine 110, which exhibited excellent spectral properties matching the optimal detection window of most fluorescence instruments. after cleavage, abs(max) is at 497 nm and em(max) is at 520 nm for rhodamine 110 [1].
References
[1] a. f. johnson, m. d. struthers, k. b. pierson, et al. nonisotopic dna detection system employing elastase and a fluorogenic rhodamine substrate. analytical chemistry 65, 2352-2359 (1993).
