Applications
The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity.
Immunogen
Anti-human IDH1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human IDH1 protein 1-414 amino acids purified from E. coli.
Background
Isocitrate Dehydrogenase is an enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+; it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle.
References
[1] Ramachandran, N. and Colman, R.F. (1980) J Biol Chem 255, 8859-64.
[2] Bennett, P.M. and Holms, W.H. (1975) J Gen Microbiol 87, 37-51.
[3] Hurley, J.H. et al. (1990) Science 249, 1012-6.
[4] Bleeker, F.E. et al. (2009) Hum Mutat 30, 7-11.
[5] Abbas, S. et al. (2010) Blood 116, 2122-6.
[6] Paschka, P. et al. (2010) J Clin Oncol 28, 3636-43.
[7] Watanabe, T. et al. (2009) Am J Pathol 174, 1149-53.
[8] Zhao, S. et al. (2009) Science 324, 261-5.
