APP antibody

Source

Rabbit

Applications

APP antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.

Reactivity

Human;Mouse;Rat;Monkey

Immunogen

Anti-human APP mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human APP amino acids 18-289 purified from E. coli.

Background

APP is a cell surface receptor and transmembrane precursor protein which is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in the APP gene are implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy).

References

[1] Selkoe, D.J. (1996) J Biol Chem 271, 18295-8.
[2] Caporaso, G.L. et al. (1992) Proc Natl Acad Sci USA 89, 3055-9.
[3] Hung, A.Y. and Selkoe, D.J. (1994) EMBO J 13, 534-42.
[4] Suzuki, T. et al. (1994) EMBO J 13, 1114-22.
[5] Ando, K. et al. (1999) J Neurosci 19, 4421-7.
[6] Iijima, K. et al. (2000) J Neurochem 75, 1085-11.