Description
Phen green SK (PGSK) diacetate is a fluorescent heavy metal indicator that reacts with a variety of metal ions, including Fe
2+, Cd
2+, Co
2+, Ni
2+, and Zn
2+.
1 PGSK diacetate displays excitation/emission maxima of 507/532 nm, respectively, and fluorescence is quenched upon interaction with metal ions. It has been used to quantify iron in isolated rat hepatocytes, characterize metal-ion selectivity of divalent metal-ion transporter 1 (DMT1), and monitor copper transport across
P. sativum chloroplast membranes.
1,2,3
References
1. Illing, A.C., Shawki, A., Cunningham, C.L., et al.
Substrate profile and metal-ion selectivity of human divalent metal-ion transporter-1 J. Biol. Chem. 287(36),30485-30496(2012).
2. Petrat, F., Rauen, U., and de Groot, H.
Determination of the chelatable iron pool of isolated rat hepatocytes by digital fluorescence microscopy using the fluorescent probe, phen green SK Hepatology 29(4),1171-1179(1999).
3. Shingles, R., Wimmers, L.E., and McCarty, R.E.
Copper transport across pea thylakoid membranes Plant Physiol. 125(1),145-151(2004).
