Uses
His.Bind(R) Resin have been used: in purification of His.Tag(R) fusion proteins by metal chelation chromatography, for purification of the bacterially produced OCRL1WT/VAR phosphatase domain to study heterogeneity in Lowe Syndromein protein purification to study anaerobic phloroglucinol degradation by Clostridium scatologenes for isolation of His6-tagged proteins for studying the role of human repressor activator protein 1 (RAP1) and glial fibrillary acidic protein (GFAPɛ)proteins in increasing γ-secretase activity in a yeast model system.
Biological Activity
His.Bind(R) resin helps in the purification of proteins containing the His-tagged sequence by the process of metal chelation chromatography. It utilizes the charge buffer provided in the His.Bind(R) Buffer Kit, th at binds the His-tagged sequence effectively with Ni2+ or Co2+ cations th at are immobilized onto the His.Bind(R) resin. The target protein can be easily recovered by eluting with imidazole. The His.Bind(R) Resin is designed to be regenerated and reused multiple times, making it a cost-effective solution. Its versatility allows for protein purification under gentle, non-denaturing conditions, or in the presence of either guanidine or urea, depending on the specific requirements of the purification process.
