GRIESS REAGENT (MODIFIED)

Uses

Griess reagent (modified) has been used:

• in the nitrite assay of primary spinal cord glial cells stimulated with supernatant of damaged sensory neuron (SDSN)
• for measuring nitrite/nitrate oxidized from nitric oxide in stimulated chondrocytes
• for measuring nitric oxide (NO) degradation in RAW264.7 macrophage cells

General Description

Griess reagent is based on Griess reaction. The components include naphthylethylenediamine dihydrochloride suspended in water and sulphanilamide in phosphoric acid. This reagent reacts with nitrite in samples to form a purple azo product, absorbance of which is measured at 546 nm.