Description
Biotin-PEG
3-azide is a PEGylated version of biotin-azide that is more hydrophilic due to the incorporation of a PEGylated spacer. It can be used to label terminal alkynes
via copper-catalyzed click reactions or copper-free click reactions with cyclooctynes., Biotin-PEG
3-azide has been used to non-enzymatically and covalently tag proteins, which can be separated and detected using common laboratory methods such as SDS-PAGE and Western blot. It can be used in combination with other protein tags. Biotin-PEG
3-azide has also been used in nanostructured biointerfaces to study ligand and receptor spacing when there are different signaling molecules present.
Uses
Biotinylation reagent for labeling alkyne containing molecules or biomolecules using either copper-catalyzed 1,3 dipolar cycloaddition click chemistry or copper-free click chemistry with cyclooctyne derivatives. The azide group reacts with alkynes to form a stable triazole linkage facilitating the introduction of biotin into your alkyne modified system of interest. This reaction is bioorthogonal or in other words compatible with biological systems in that its components do not react with the biological environment.
reaction suitability
reaction type: click chemistry
References
[1] FRANZISKA C. SCHENK. Dual-Functionalized Nanostructured Biointerfaces by Click Chemistry[J]. Langmuir, 2014, 30 23: 6897-6905. DOI:
10.1021/la500766t[2] HIROSHI NONAKA. Non-enzymatic Covalent Protein Labeling Using a Reactive Tag[J]. Journal of the American Chemical Society, 2007, 129 51: 15777-15779. DOI:
10.1021/ja074176d[3] DR. MATTHEW LLUIS. Characterization of C-Alkyl Amidines as Bioavailable Covalent Reversible Inhibitors of Human DDAH-1[J]. ChemMedChem, 2010, 6 1: 81-88. DOI:
10.1002/cmdc.201000392
