Description
DNP-INT is a quinone analog that inhibits electron transport in plants by competitively inhibiting plastoquinol oxidation by binding at the Q
o site of cytochrome
b6f (K
d = 1.4 nM).
1 It inhibits electron flow from water to NADP or methylviologen by 50 and 100% when used at concentrations of 0.5 or 5 μM, respectively.
2,3
References
1. Barbagallo, R.P., Finazzi, G., and Forti, G.
Effects of inhibitors on the activity of the cytochrome b6f complex: Evidence for the existence of two binding pockets in the lumenal site Biochemistry 38(39),12814-12821(1999).
2. Trebst, A., Wietoska, H., Draber, W., et al.
The inhibition of photosynthetic electron flow in chloroplasts by the dinitrophenylether of bromo-or iodo-nitrothymol Z. Naturforsch. C J. Biosci. 33(11-12),919-927(1978).
3. Malkin, R.
Interaction of stigmatellin and DNP-INT with the Rieske iron-sulfur center of the chloroplast cytochrome b6f complex. FEBS Lett. 208(2),317-320(1986).
