Uses
The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.
Uses
Anti-DNP antibody produced in rabbit was used to tag derivatized protein carbonyl groups in plasma by ELISA. It was used for differential cell count of zona-free blastocysts in IVF embryos.
General Description
Dinitrophenyl (DNP) is a hapten that is often used for labeling primary or secondary probes in immunological assays. Hence, anti-DNP antibody is a useful tool for the detection and analysis of target molecules. Monoclonal Anti-DNP antibody is specific for DNP.
IgE gene is encoded on human chromosome 14. IgE is the least circulating antibody, but critical for eliciting hypersensitivity reaction against allergens.
Biochem/physiol Actions
IgE is produced by B cells by class switch recombination in response to allergens. IgE is also associated with mast cells and is abundantly produced in response to allergens especially in asthma and allergic rhinitis. IgE regulates mast cell homeostasis and is upregulated in food allergy. IgE is implicated in the pathogenesis of autoimmune diseases like rheumatoid arthritis and systemic lupus erythematosus. IgE levels are modulated by other genes present on chromosome 14 especially in patients with asthma. Patients with mutations in signal transducer and activator of transcription 3 (STAT3) are associated high levels of IgE called the hyper–immunoglobulin E syndrome (HIES).
