Uses
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) has been used as a peroxidase substrate in ELISA experiment. This substrate gives a green color soluble end product. It has also been used as a colloidal preparation spread over the standard media plates, to evaluate the lignin oxidation activity. Presence of green coloration of the substrate exhibits microbial laccase activity.
General Description
The product is supplied as a ready-to-use one component substrate for peroxidase consisting 2,2′-azino-bis (3-ethylbenz-thiazoline-6-sulfonic acid) in an acidic buffer. The substrate is colorless before reacting with peroxidase and produces a blue-green color reaction product after reacting with it. 1% sodium dodecyl sulfate (SDS) may be used to stop the reaction during end-point assays. Since this substrate produces a soluble reaction product, it is not recommended for immunoblotting or histochemical applications.
Biochem/physiol Actions
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) is a cation and undergoes oxidation in the presence of hydrogen peroxide. The oxidized ABTS is a blue-green product and is measurable colorimetrically. A combination of ABTS, hydrogen peroxide and myoglobin is a standard system employed for measuring total antioxidant capacity(TAC) from samples. ABTS method is flexible at varying pH and most widely used. ABTS is a substrate for phenol oxidase enzyme.
