Description
Cayman’s Thioredoxin Reductase Colorimetric Assay Kit provides a convenient method for detecting TrxR activity in tissue homogenates and cell lysates. It is based on the reduction of DTNB (5,5’-dithio-bis(2-dinitrobenzoic acid); Ellman’s reagent) with NADPH to 5-thio-2-nitrobenzoic acid (TNB) which produces a yellow product that is measured at 405 nm.1,2 The kit includes all reagents needed to assay mammalian TrxR activity. Measurement of TrxR activity by DTNB reduction in the absence and in the presence of aurothiomalate, a specific TrxR inhibitor included in the kit, allows for correction of non-thioredoxin reductase-independent DTNB reduction (i.e., presence of glutathione).3 The difference between the two results is the DTNB reduction due to TrxR activity.Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.WARNING This product is not for human or veterinary use.
