Uses
The M30 CytoDEATH antibody is used for the determination of early apoptotic events in cells and tissue sections by detection of a specific epitope of cytokeratin 18 that is presented after cleavage by caspases. Detect apoptosis by applying the M30 antibody to fixed samples, then using secondary detection systems suitable for immunohistochemistry, immunocytochemistry, and flow cytometry.
General Description
Mouse monoclonal antibody (Clone M30, mouse IgG
2b) is used for the detection of apoptosis in epithelial cells (caspase cleavage product of cytokeratin 18). It is provided in lyophilized, stabilized, formalin grade form.
During apoptosis, vital intracellular proteins are cleaved. The proteases that mediate this process are called caspases (Cysteinyl-aspartic acid proteases). Caspases are expressed as zymogenes, which are activated by different apoptosis inducers. Once activated, a single caspase activates a cascade of caspases. Until recently, cytokeratins, in particular cytokeratin 18 (CK18), were not known to be affected by early events of apoptosis. Recently, it has been shown that the M30 antibody recognizes a specific caspase-cleavage site within cytokeratin 18, which is not detectable in native CK18 of normal cells. Consequently, the M30 CytoDEATH antibody is a unique tool for the easy and reliable determination of very early apoptotic events in single cells and tissue sections.
- Assay time: 2 hours for immunofluorescence on cells, 3.5 hours for staining of tissues (excluding dewaxing)
- Sample material: Adherent cells, tissue samples (routinely fixed and paraffin-embedded tissue sections, cryostat sections)
