Description
Prostaglandin E
2 (PGE
2) is the major eicosanoid product of cyclooxygenase-
1 and 2 in epithelial cells, leukocytes, reproductive tissues, and many others. PGE
2 contracts smooth muscle, protects the gastric mucosa, modulates immune function, and regulates fertility and implantation. PGE
2 acts through at least four 7-
transmembrane G
protein-coupled receptors, EP
1-EP
4. PGE
2-
biotin is an affinity probe which allows PGE
2 to be detected through an interaction with the biotin ligand. PGE
2-
biotin was designed to allow PGE
2 to be detected in complexes with transmembrane receptors and/or nucleic acid or protein binding partners. It is thus a tool to be used in the general elucidation of the mechanism of action of PGs.