Description
Methyl Green, Zinc chloride salt (chemical formula: C27H35Cl4N3Zn) is the zinc chloride salt form of Methyl green. Methyl green is a cationic or positive charged stain. Since it can binds selectively to the AT-rich regions of native DNA, it is mainly used for the DNA and RNA staining in the cell nuclei. Methyl Green, Zinc chloride Salt, as a CERTISTAIN® dry dye for DNA staining, it has many application: used as a tracking dye in acid buffers for electrophoresis; used to demonstrate DNA and RNA in tissues sections in the Methyl Green-Pyronin method; used as an exclusion viability stain for cells. In addition, it can also be used in fluorimetric assay and flow cytometry.
References
http://www.sigmaaldrich.com/catalog/product/sigma/67060?lang=zh®ion=CN
https://pubchem.ncbi.nlm.nih.gov/compound/3084002#section=Canonical-SMILES
https://en.wikipedia.org/wiki/Methyl_green
http://www.abcam.com/methyl-green-zinc-chloride-salt-ab146287.html
Chemical Properties
maroon to reddish-brown crystals
Uses
Methyl Green is a DNA stain used as a tracking dye in acid buffers for electrophoresis. It helps differentiate bacteria and is also a tissue stain for nucleoli and mitochondria in tissues. It is used with Bismarck Brown frequently.
Purification Methods
Crystallise the dye from hot water. [Beilstein 13 IV 2286.]
